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| Rapid Identification of Constituents in Keke Tablets by High-performance Liquid Chromatography－Quadrupole Time-of-flight Mass Spectrometry |
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| DOI： |
| KeyWord:Keke Tablets high-performance liquid chromatography－quadrupole time-of-flight mass spectrometry（UPLC－Q－TOF MS） material basis qualitative analysis |
| Author | Institution |
| ZHOU Xi，WU Hui-qin，LUO Hui-tai，LAI Xiao-na，HUANG Fang，PENG Li-hua |
1. Guangdong Provincial Engineering Research Center for Quality and Safety of Traditional Chinese Medicine，Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology， Institute of Analysis，Guangdong Academy of Sciences（China National Analytical Center， Guangzhou），Guangzhou ，China; 2. Zhongshan Zhongzhi Pharmaceutical Group Co.，Ltd.，Zhongshan ，China |
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| Abstract: |
| A high-performance liquid chromatography－quadrupole time-of-flight mass spectrometric（UPLC－Q－TOF MS） method was established to identify the constituents in Keke Tablets．The analytes was separated on a waters CORTECS T3 column（150 mm × 2.1 mm，1.6 μm） by gradient elution，with 0.1% formic acid water－acetonitrile as the mobile phase，and then analyzed in both positive and negative ions modes．Constituents in methanol－H2O extract of Keke tablets were identified by mass spectrometry database，fragmentation regularities of mass spectra，consulting literature reports，retention time and mass data of reference compounds．A total of 94 constituents were identified，including 33 alkaloids，22 flavones，9 phenylpropanoids，8 triterpenoid saponins，5 amino acids，3 cyanophoric glycosides，6 organic acids and 8 other compounds．Chemical constitutions of Keke tablets were comprehensively analyzed，which will be helpful to the quality control and pharmacodynamics material basis research. |
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