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| Determination of 12 Pyrethroid Pesticides in Blood and Liver by UPLC－QE－Orbitrap HRMS |
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| DOI： |
| KeyWord:ultra performance liquid chromatography－quadrupole/electrostatic field orbittrap high-resolution mass spectrometry（UPLC－QE－Orbitrap HRMS） pyrethroids blood liver detection |
| Author | Institution |
| ZHANG He-yao，GUO Fei，JIA Zhen-jun，LI Peng |
1. School of Investigation，People’s Public Security University of China，Beijing ，China; 2. Judicial Appraisal Center of Zibo Public Security Bureau Organization-Company，Zibo ，China; 3. School of Forensic Medicine，Shanxi Medical University，Taiyuan ，China |
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| Abstract: |
| A method for the determination of 12 pyrethroid pesticides in blood and liver by ultra performance liquid chromatography－quadrupole/electrostatic field orbittrap high-resolution mass spectrometry（UPLC－QE－Orbitrap HRMS） was developed．After acetonitrile precipitation，the blood and liver were separated by gradient elution on a Hypersil GOLD VANQUISH analytical column（1.9 μm，2.1 mm × 100 mm） with 5 mmol/L ammonium acetate（containing 0.1% formic acid） and methanol as mobile phase．Full scan and automatic trigger secondary mass spectrometry（Full MS/dd－MS2） were used for detection．The experimental results showed that the linearities of 12 pyrethroid pesticides were good in their linear range（r2 ≥ 0.990 4）．The limits of detection in blood and liver were in the range of 1－18 ng/mL and 1－18 ng/g，respectively．The limits of quantitation were 2－20 ng/mL and 2－20 ng/g，respectively．The matrix effects were 82.3%－117%，and the recoveries were 74.1%－120%．The intra-day RSD（intra-RSD） were not more than 11%，and inter-day RSD（inter-RSD） were not more than 12%．This method could meet the requirements of public security organs in handling actual cases and was suitable for the detection and identification of pyrethroid pesticides in blood and liver samples. |
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