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| Detection of Glucose and Acid Phosphatase Using a Multifunctional Enzyme Cascade Sensing System Based on Hg2［Fe（CN）6］ Nanozyme |
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| DOI： |
| KeyWord:nanozyme enzyme cascade system Hg2［Fe（CN）6］ glucose acid phosphatase（ACP） |
| Author | Institution |
| GUO Ao-huan，LI Ping，WANG Guang-li |
School of Chemical and Material Engineering，Jiangnan University，Wuxi ，China |
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| Abstract: |
| According to the formation of Hg2［Fe（CN）6］ nanozyme based on the in-situ reaction of Hg2+ and K4［Fe（CN）6］，a multifunctional biosensing platform was constructed based on an enzyme cascade in which the activity of Hg2［Fe（CN）6］ depended on hydrogen peroxide（H2O2），as well as pyrophosphate（PPi） was able to bind to Hg2+ and prevent the formation of Hg2［Fe（CN）6］ nanozyme．In the presence of glucose，glucose oxidase oxidized glucose to generate H2O2，and then Hg2［Fe（CN）6］ catalyzed the decomposition of H2O2 to produce hydroxyl radicals（·OH） to oxidize the substrate of 3，3'，5，5'-tetramethylbenzidine（TMB），which enabled the detection of glucose．The linear range of the method for glucose detection was 0.10－500 μmol/L，and the detection limit was 30 nmol/L．In the presence of acid phosphatase（ACP），ACP could hydrolyze PPi to release Hg2+ that could otherwise be bound by PPi，which allowed the reaction of K4［Fe（CN）6］ with Hg2+ to generate Hg2［Fe（CN）6］ nanozyme for catalyzing the oxidation of TMB．Results showed that the linear range of the method for the detection of ACP was 0.03－50 U/L，and the detection limit was 0.007 0 U/L．This sensing platform is different from the traditional detection system for single target，which provides a new idea for the design of multifunctional sensing systems with simple operation and high sensitivity. |
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