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| Metabolomic Investigation on Serum of Gout Patients Based on Ultra-high Performance Liquid Chromatography－Quadrupole Time-of-flight Mass Spectrometry |
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| DOI： |
| KeyWord:gout metabolomics UPLC－Q/TOF MS biomarkers serum |
| Author | Institution |
| TIAN Li，YANG Bao，HU Xue-li，DU Gui-yan，TANG Sheng-yao，LIU Guo-sheng |
1. Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases（Hubei Minzu University），Enshi ，China; 2. Chinese Medicinal Materials Products Quality Supevision and Inspection Center in Wuling Mountainous Area，Hubei Minzu University，Enshi ，China; 3. Medical School， Hubei Minzu University，Enshi ，China; 4. Clinical Laboratory，Enshi City Maternal and Child Health Centre，Enshi ，China; 5. Department of Medical Laboratory，Affiliated Minda Hospital of Hubei University for Nationalities，Enshi ，China |
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| Abstract: |
| An ultra-high performance liquid chromatography－quadrupole time-of-flight mass spectrometry（UPLC－Q/TOF MS） was applied to establish a metabolic map on the serum samples of 60 healthy volunteers（control group） and 65 gout patients（gout group），to investigate the serum metabolomics differences between patients suffering from gout and healthy volunteers to find potential serum biomarkers．The obtained data were pattern-identified based on principal component analysis and orthogonal partial least squares analysis．The serum metabolic markers for gout patients were screened by the combination of variable weight projection analysis and a volcanic map．Through data analysis and database search，a total of 63 different metabolites were screened，27 metabolites were significantly up-regulated（P < 0.05），while 36 metabolites were significantly down-regulated（P < 0.05），mainly including glycerol phospholipids，amino acids and choline，etc．Firstly，conducting ROC curve analysis on the obtained differential metabolites，14 metabolites with AUC area greater than 0.8 were the better metabolites for diagnosis．Then，63 differential metabolites screened were analyzed for metabolic pathway enrichment．Impact > 0.1 and P < 0.05 were taken as the standards，the obtained metabolic pathway primarily included glycerol phospholipid metabolism，ether lipid metabolism，linoleic acid metabolism，cysteine and methionine metabolism，arachidonic acid metabolism and the mutual conversion of pentasaccharide and glucuronic acid．Results showed that there were apparent differences in serum metabolism levels between gout patients and healthy control populations，and the identification of differential metabolites provided an experimental basis for the pathogenesis and early screening of gout. |
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