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| Simultaneous Determination of 9 Self-heating Compounds Including Capsaicin in Cosmetics by High Performance Liquid Chromatography |
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| DOI： |
| KeyWord:self-heating compounds cosmetics high performance liquid chromatography（HPLC） |
| Author | Institution |
| WU Zhi-shan，WU Jiao-jiao，YIN Wei-cheng，YIN Shuai，LI Zhuo-fang，JIANG Cheng-jun |
1. Hunan Institute for Drug Control，Changsha ，China; 2. Food and Drug Inspection in Yueyang，Yueyang ，China |
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| Abstract: |
| A high performance liquid chromatographic（HPLC） method was established for the determination of 9 self-heating compounds，including capsaicin in cosmetics．The samples were ultrasonically extracted with 80% methanol，and then filtered．The target compounds were separated on a SymmetryShieldTMRP18（250 mm × 4.6 mm，5 μm） with a mobile phase consisting of acetonitrile and 0.5% formic acid by gradient elution，and analyzed by HPLC with a PDA detector．Under the optimal conditions，good linear relationships were obtained for nine compounds in the concentration range of 0.2－100 μg/mL，with correlation coefficients（r2） not less than 0.999 5．The limits of detection（LODs） were in the range of 0.001 0－0.003 4 mg/kg．The spiked recoveries for water，lotion and cream matrixes at three levels of 0.004，0.02，1 mg/kg ranged from 75.6% to 115%，with relative standard deviations（RSDs，n = 3） of 0.083%－7.0%．This method is accurate and sensitive，and is suitable for the qualitative and quantitative analysis of 9 self-heating compounds in cosmetics. |
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